Bioimaging

Orthogonal Measurements

Ongoing

Definitions: Orthogonal measurements: Measurements that use different physical principles to measure the same property of the same sample with the goal of minimizing method-specific biases and interferences. Complementary measurements: Measurements that corroborate each other to support the same

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Ongoing

The current gold standard for cell viability measurements is the trypan blue assay. Trypan blue dye enters dead cells that have a ruptured membrane. As a result, the dead cells are stained blue. Live cells with an intact membrane, on the other hand, do not absorb the dye molecules and remain

Automated Live Cell Imaging for Quantification of Catalytic Rates in Individual Cells

Completed

In this study, the rates at which large numbers of individual living cells enzymatically degrade green fluorescent protein (GFP) was examined using automated fluorescence microscopy. A clonal population of NIH3T3 fibroblasts that resulted from stable transfection with a construct containing the

Label-free imaging of cells and their extracellular matrix by SPR imaging

Ongoing

Cellular remodeling of their neighboring environment, extracellular matrix (ECM), is an important biological process from development biology, to wound healing, to diseases and cancers – this is a challenging process to measure and quantify. Surface plasmon resonance imaging (SPRI) has been

News and Updates

A hand in a purple glove turns a metal dial on a plate holding tiny vials of purple liquid.

Bioimaging

The Research

Orthogonal Measurements

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Automated Live Cell Imaging for Quantification of Catalytic Rates in Individual Cells

Label-free imaging of cells and their extracellular matrix by SPR imaging

News and Updates

NIST Develops New Nondestructive Method for Assessing Bioengineered Artificial Tissues

A Recap of TMAP

NIST Scientists and Engineers Pitch Innovations to Venture Capitalists