Caliskan, G.
, Kisliuk, A.
, Tsai, A.
, Soles, C.
and Sokolov, A.
(2003),
Protein Dynamics in Viscous Solvents, Journal of Chemical Physics
(Accessed May 14, 2025)
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Protein Dynamics in Viscous Solvents
Published
Author(s)
G Caliskan, A Kisliuk, , , Alexei Sokolov
Abstract
The principals governing the effective stabilization of tissues and drugs are unclear and effective agents for preservation are discovered largely by trail and error. We explore the use of low frequency Raman spectroscopy as an effective tool fro direct evaluating the ability of different solvents to suppress the conformational fluctuations that can lead to both protein activity and denaturation. We demonstrate that while trehalose provides superior stabilization at high temperatures, glycerol is more effective at suppressing protein dynamics at low temperatures. The presented results demonstrate that viscosity of the solvent is not the only parameter important for biopreservation.Citation
Journal of Chemical Physics
Volume
118
Issue
No. 9
Pub Type
Journals
Keywords
cryopreservation, glycerol, lysozyme, myoglobin, protein stability, Raman scattering, trehalose
Citation
Issues
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Created February 28, 2003, Updated October 12, 2021