U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Real-Time Measurement of Spontaneous Antigen-Antibody Dissociation

Published

Author(s)

S Kulin, Rani B. Kishore, Joseph B. Hubbard, Kristian Helmerson

Abstract

We report observation of real-time adhesion between a monoclonal IgE antibody and its specific DNP antigen. Both molecules are immobilized on the surfaces of different polystyrene microspheres trapped by optical tweezers. Monitoring spontaneous, thermally driven, successive attachment and detachment events allows a direct determination of the reaction limited detachment rate for a single bond and also for multiple bonds.
Citation
Biophysical Journal
Volume
83
Issue
No. 4
Pub Type
Journals

Keywords

antigen, biological adhesion, IgE, optical tweezers, spontaneous dissociation

Citation

Kulin, S. , Kishore, R. , Hubbard, J. and Helmerson, K. (2002), Real-Time Measurement of Spontaneous Antigen-Antibody Dissociation, Biophysical Journal (Accessed May 12, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created October 1, 2002, Updated February 17, 2017