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Health / Clinical diagnostics

Medical imaging

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Displaying 151 - 175 of 229

3D Segmentation of Stem Cells from Thousands of Confocal Microscopy Images

March 1, 2016
Author(s)
Peter Bajcsy, Mylene H. Simon, Stephen J. Florczyk, Carl G. Simon Jr., Derek Juba, Mary C. Brady
We address the problem of estimating 3D segmentation performance when segmentation is applied to thousands of confocal microscopy images (z-stacks) of cells. With a very large number of z-stacks, manual inputs to validate each segmentation result are

Design of a breast phantom for quantitative MRI

January 1, 2016
Author(s)
Kathryn E. Keenan, Sheye O. Aliu, Lisa J. Wilmes, David C. Newitt, Ella F. Jones, Wen Li, Michael A. Boss, Nola M. Hylton
Purpose: We present a breast phantom designed to enable quantitative assessment of measurements of T 1 relaxation time, apparent diffusion coefficient (ADC), and other attributes of breast tissue, with long-term support from a national metrology institute

Beam-Scanning for Rapid Coherent Raman Hyperspectral Imaging

December 14, 2015
Author(s)
Seungwan Ryu, Charles Camp, Ying Jin, Marcus T. Cicerone, Young Jong Lee
Coherent Raman imaging requires high peak power laser pulses to maximize the nonlinear multiphoton signal generation, but accompanying photo-induced sample damage often poses a challenge to microscopic imaging studies. We demonstrate that a beam-scanning

Quantitative scheme for full-field polarization rotating fluorescence microscopy (PROM) using a liquid crystal variable retarder

May 14, 2015
Author(s)
John F. Lesoine, Ji Y. Lee, Hyeong G. Kang, Matthew L. Clarke, Robert C. Chang, Ralph Nossal, Jeeseong C. Hwang
We introduce real-time, full-field, polarization rotating fluorescence microscopy (PROM) to monitor the absorption dipole orientations of fluorescent molecules. A quarter-wave plate, in combination with a liquid crystal variable retarder (LCVR), provides a

Evaluation of Low-Contrast Detectability of Iterative Reconstruction across Multiple Institutions, CT Scanner Manufacturers, and Radiation Exposure Levels

February 19, 2015
Author(s)
Adele P. Peskin, Ganesh Saiprasad, James J. Filliben, Alden A. Dima
Purpose: To evaluate the impact of Iterative Reconstruction (IR) compared with Filtered Back Projection (FBP) using low-contrast objects in phantom images across vendors and exposure levels. Materials and Methods: Randomized repeat scans of the Gammex 464

High Resolution Surface Plasmon Resonance Imaging for Single Cells

December 1, 2014
Author(s)
Alexander W. Peterson, Michael W. Halter, Alessandro Tona, Anne L. Plant
Background Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However

Automated Ranking of Stem Cell Colonies by Translating Biological Rules to Computational Models

September 20, 2014
Author(s)
Adele P. Peskin, Steven P. Lund, YaShian Li-Baboud, Michael W. Halter, Anne L. Plant, Peter Bajcsy
This paper addresses the problem of automating an image ranking process for stem cell colonies. We automate the manual process in a novel way: instead of fitting off-the-shelf image features and colony ranks to prediction models, we define a new feature

Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass

August 11, 2014
Author(s)
Michael W. Halter, Elianna Bier, Paul C. DeRose, Gregory A. Cooksey, Steven J. Choquette, Anne L. Plant, John T. Elliott
Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist for

High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues

July 20, 2014
Author(s)
Charles H. Camp, Young J. Lee, John M. Heddleston, Christopher M. Hartshorn, Angela R. Hight Walker, Jeremy N. Rich, Justin D. Lathia, Marcus T. Cicerone
We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique configuration of laser

Prototype Phantoms for Characterization of Ultra-Low Field Magnetic Resonance Imaging

November 26, 2013
Author(s)
Michael A. Boss, John A. Mates, Sarah E. Busch, Paul SanGiorgio, Stephen E. Russek, Kai Buckenmaier, Kent D. Irwin, Hsiao-Mei Cho, Gene C. Hilton, John Clarke
Purpose: Prototype phantoms were designed, constructed, and characterized for the purpose of calibrating ultralow field magnetic resonance imaging (ULF MRI) systems. The phantoms were designed to measure spatial resolution and to quantify sensitivity to