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Displaying 351 - 375 of 482

Development of Y STR Megaplex Assays

December 1, 2001

Author(s)

R Schoske, John Butler, Peter Vallone, Margaret C. Kline, M. Prinz, A J. Redd, M F. Hammer

Y Chromosome short tandem repeat markers have a number of applications in human identity testing including typing the perpetrator of sexual assault cases without differential extraction and tracing paternal lineages for missing person investigations. In

Substrate Specificity and Excision Kinetics of Escherichia Coli Endonuclease VIII (Nei) for Modified Bases in DNA Damaged by Free Radicals

October 9, 2001

Author(s)

M. Dizdaroglu

Endonuclease VIII (Nei) is one of three enzymes in Escherichia coli that are involved in base-excision repair of oxidative damage to DNA. We investigated the substrate specificity and excision kinetics of this DNA glycosylase for bases in DNA that have

Improved Analysis of DNA Short Tandem Repeats With Time-of-Flight Mass Spectrometry

October 1, 2001

Author(s)

John M. Butler, C. H. Becker

Eukaryotic-Like Adenylyl Cyclases in Mycobacterium Tuberculosis H37Rv - Cloning and Characterization

September 14, 2001

Author(s)

Sathyavelu K. Reddy, M A. Kamireddi, K. Dhanireddi, L. Young, A M. Davis, Prasad T. Reddy

We have cloned a cya gene from a human pathogen Mycobacterium tuberculosis H37Rv by complementation of catabolic defect. (Accession No. AF017731, 1977). M. tuberculosis H37Rv genome sequence revealed five genes (Rv1264, Rv1318c, Rv1319c, Rv1320c, and

Calculating sequence-dependent melting stability of duplex DNA oligomers and multiplex sequence analysis by graphs

September 1, 2001

Author(s)

A. S. Benight, P. Pancoska, R. Owczarzy, Peter M. Vallone, J. Nesetril, P. V. Riccelli

Effect of Single Mutations on the Specificity of Esherichia Coli FPG Protein for Excision of Purine Lesions From DNA Damaged By Free Radicals

September 1, 2001

Author(s)

O. Sidorkina, M. Dizdaroglu, J. Laval

The Formamidopyrimidine N-DNA glycosylase (Fpg protein) of Escherichia coli is a DNA repair enzyme that is specific for the removal of purine-derived lesions from DNA damaged by free radicals and other oxidative processes. We investigated the effect of

Measurement of 8-Hydroxy-2'-Deoxyadenosine In DNA by Liquid Chromatography/Mass Spectrometry

August 1, 2001

Author(s)

Pawel Jaruga, H Rodriguez, M Miral Dizdar

8-Hydroxyadenine (8-OH-Ade) is one of the major lesions, which is formed in DNA by hydroxyl radical attack on the C-8 position of adenine followed by oxidation. We describe the measurement of the nucleoside form of this compound, 8-hydroxy-2'

Controlling Electrophoretic Trapping of Circular DNA by Addition of Starch Preparations to Agarose Gels

July 1, 2001

Author(s)

Kenneth D. Cole, C M. Tellez-Rodriguez, R Nguyen

Detection of Viable Cryptosporidium Parvum Using DNA-Modified Liposomes in a Microfluidic Chip

July 1, 2001

Author(s)

M. B. Esch, Laurie E. Locascio, Michael J. Tarlov, R. A. Durst

This paper describes a microfluidic chip that enables the detection of viable Cryptosporidium parvum by detecting RNA amplified by nucleic-acid sequence-based amplification (NASBA). The mRNA serving as the template for NASBA is produced by viable C. parvum

Quality Control of PCR Primers Used in Multiplex STR Amplifcation Reactions

June 1, 2001

Author(s)

John M. Butler, J E. Devaney, M A. Marino, Peter M. Vallone

Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification

The Application of Capillary Electrophoresis in the Analysis of PCR Products Used in Genetic Typing

May 15, 2001

Author(s)

B. R. McCord, John M. Butler

Measurement and Application of 1 H- 19 F Dipolar Couplings in the Structure Determination of 2'-Fluorolabeled RNA

May 1, 2001

Author(s)

B. Luy, John Marino

Residual dipolar couplings can provide powerful restraints for determination and refinement of the solution structure of macromolecules. The application of these couplings in nucleic acid structure elucidation can have an especially dramatic impact since

Identification and Quantification of 8,5'-Cyclo-2'-Deoxyadenosine in DNA by Liquid Chromatography/Mass Spectrometry

April 1, 2001

Author(s)

M. Dizdaroglu, Pawel Jaruga, H Rodriguez

Recent studies suggested that 8,5'-cyclo-2'-deoxyadenosine may play a role in diseases with defective nucleotide-excision repair. This compound is one of the major lesions formed in DNA by hydroxyl radical attack on sugar moiety of 2'-deoxyadenosine. It is

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