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Displaying 51 - 75 of 482

DNA Error Rates

December 7, 2016
Author(s)
John Butler
The 1993 U.S. Supreme Court ruling in Daubert vs. Merrell-Dow Pharmaceuticals insists on knowledge regarding error rates and their application when evidence is introduced into court [1]. The 1996 National Research Council (NRC II) report The Evaluation of

DNA Commission of the International Society for Forensic Genetics: Recommendations on the validation of software programs performing biostatistical calculations for forensic genetics applications

September 4, 2016
Author(s)
Michael D. Coble, John S. Buckleton, John M. Butler, Barbara Guttman
The use of biostatistical software programs to assist in data interpretation and calculate likelihood ratios is essential to forensic geneticists and part of the daily case work flow for both kinship and DNA identification laboratories. Previous

Evaluation of Forensic DNA Mixture Evidence: Guidelines for Evaluation, Interpretation, and Statistical Calculations using the Combined Probability of Inclusion

August 31, 2016
Author(s)
Frederick R. Bieber, John Buckleton, Bruce Budowle, John Butler, Michael D. Coble
The evaluation and interpretation of forensic DNA mixture evidence faces greater interpretational challenges due to increasingly complex mixture evidence. Such challenges include: casework involving low quantity or degraded evidence leading to allele and

Is the Factor of 10 Still Applicable Today?

July 13, 2016
Author(s)
Simone N. Gittelson, John S. Buckleton
The assignment of the weight of DNA evidence depends on a number of factors (allele probability estimates, the population genetic model used, the value of the coancestry coefficient, etc.). One of these factors is the allele probability estimates from a

Expected net gain data of low-template DNA analyses

May 30, 2016
Author(s)
Simone N. Gittelson, Carolyn R. Steffen, Michael D. Coble
Low-template DNA analyses are affected by stochastic effects which can produce a configuration of peaks in the electropherogram (EPG) that is different from the genotype of the DNA's donor. A probabilistic and decision-theoretic model can quantify the

Low-template DNA: A single DNA analysis or two replicates?

April 18, 2016
Author(s)
Simone N. Gittelson, Carolyn R. Steffen, Michael D. Coble
This study investigates the following two questions: (i) Should the DNA analyst concentrate the DNA extract into a single amplification or should he/she split it up to do two replicates? (ii) Given the electropherogram obtained from a first analysis, is it

Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

January 24, 2016
Author(s)
Sandra M. Da Silva, Lindsay Harris, Nathanael David Olson, Steven Lund, Autumn S. Downey, Zvi Kelman, Marc L. Salit, Jayne D. Morrow
Aims: We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR). Methods and results

Sequence variation of 22 autosomal STR loci detected by next generation sequencing

December 1, 2015
Author(s)
Katherine Gettings, Kevin M. Kiesler, Seth A. Faith, Elizabeth Montano, Christine H. Baker, Brian A. Young, Richard A. Guerreri, Peter Vallone
Sequencing short tandem repeat (STR) loci allows for determination of repeat motif variations within the STR (or entire PCR amplicon) which cannot be ascertained by size-based PCR fragment analysis. Sanger sequencing has been used in research laboratories
Displaying 51 - 75 of 482