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Search Publications by: John Butler (Fed)

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Displaying 176 - 200 of 203

Polymerase Chain Reaction Amplification of DNA From Aged Blood Stains: Quantitative Evaluation of the Suitability for Purpose of Four Filter Papers as Archival Media

April 15, 2002
Author(s)
Margaret C. Kline, David L. Duewer, Janette W. Redman, John M. Butler, D A. Boyer
In collaboration with the Armed Forces Institute of Pathology's Department of Defense DNA Registry, the National Institute of Standards and Technology recently evaluated the performance of a short tandem repeat (STR) multiplex with dried whole blood stains

General Strategy for the Design of Multiplex PCR Assays Involving Y STR Markers

January 1, 2002
Author(s)
R Schoske, Peter Vallone, C M. Ruitberg, John Butler
The simultaneous amplification of multiple regions of a DNA template is routinely performed using the Polymerase Chain Reaction (PCR) in a process termed multiplex PCR. A useful strategy involving the design, testing and optimization of multiplex PCR

Development of Y STR Megaplex Assays

December 1, 2001
Author(s)
R Schoske, John Butler, Peter Vallone, Margaret C. Kline, M. Prinz, A J. Redd, M F. Hammer
Y Chromosome short tandem repeat markers have a number of applications in human identity testing including typing the perpetrator of sexual assault cases without differential extraction and tracing paternal lineages for missing person investigations. In

Quality Control of PCR Primers Used in Multiplex STR Amplifcation Reactions

June 1, 2001
Author(s)
John M. Butler, J E. Devaney, M A. Marino, Peter M. Vallone
Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification

Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions

February 1, 2001
Author(s)
John M. Butler, C M. Ruitberg, Peter M. Vallone
Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining