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Search Publications by: Jeeseong C. Hwang (Fed)

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Displaying 76 - 100 of 171

Designing Microarrays Phantoms for Hyperspectral Imaging Validation

May 9, 2012
Author(s)
Matthew L. Clarke, Ji Y. Lee, Daniel V. Samarov, David W. Allen, Maritoni A. Litorja, Ralph Nossal, Jeeseong Hwang
The simple design of custom-tailored microarrays for the characterization of hyperspectral imaging systems and analysis methods for biologically relevant samples. Individual and mixtures of dyes were printed on microscope slides and imaged by a

Hyperspectral Image Projector Applications

February 15, 2012
Author(s)
Joseph P. Rice, Steven W. Brown, David W. Allen, Howard W. Yoon, Maritoni A. Litorja, Jeeseong Hwang
For the past several years NIST has been developing, along with several collaborators, a Hyperspectral Image Projector (HIP). This scene projector produces high-resolution programmable spectra and projects them into dynamic two-dimensional images. The

Validating the LASSO Algorithm by Unmixing Spectral Signatures in Multicolor Phantoms

February 1, 2012
Author(s)
Daniel V. Samarov, Matthew L. Clarke, Ji Y. Lee, David W. Allen, Maritoni A. Litorja, Jeeseong Hwang
As hyperspectral imaging (HSI) sees increased implementation into the biological and medical fields it becomes increasingly important that the algorithms being used to analyze the corresponding output be validated. While certainly important under any

Hyperspectral Imaging and Analysis of Single Erythrocytes

August 12, 2011
Author(s)
Ji Y. Lee, Matthew L. Clarke, Fuyuki Tokumasu, John F. Lesoine, David W. Allen, Robert C. Chang, Maritoni A. Litorja, Jeeseong Hwang
We report a hyperspectral imaging and analysis technique to resolve unique physico-chemical characteristics of subcellular substances in single erythrocytes. We constructed a microscope system installed with a spectral light engine capable of controlling

Real-time polarization microscopy for probing local distributions of biomolecules

February 28, 2011
Author(s)
Jeeseong Hwang, Ji Y. Lee, John F. Lesoine, Hyeong G. Kang, Matthew L. Clarke, Robert C. Chang
We present real-time, full-field, fluorescence polarization microscopy and its calibration and validation methods to monitor the absorption dipole orientation of fluorescent molecules. A quarter-wave plate, in combination with a liquid crystal variable

Characterization of Hyperspectral Imaging and Analysis via Microarray Printing of Dyes

February 22, 2011
Author(s)
Matthew L. Clarke, Maritoni A. Litorja, David W. Allen, Daniel V. Samarov, Jeeseong Hwang
The application of hyperspectral imaging requires rigorous characterization of the spatial and spectral imaging domains of the system. We present a microarray printing methodology for the testing of absorption or reflectance microscopy measurements. This

System-independent assessment of OCT axial resolution with a bar chart phantom

February 10, 2011
Author(s)
Robert C. Chang, Jeeseong Hwang, Christopher M. Stafford, Anant Agrawal, T. Joshua Pfefer, Megan Connors
We present a novel optical phantom approach for the characterization of OCT axial resolution and contrast via multilayered “bar charts.” We explored two methods to fabricate these phantoms: the first is based on a combinatorial methods approach from

Structural Analysis of Soft Multicomponent Nanoparticle Clusters Using Electrospray Differential Mobility Analysis with Transmission Electron Microscopy

October 22, 2010
Author(s)
Leonard F. Pease , Jeremy Feldblyum, Silvia H. DePaoli Lacaerda, Rajesekhar Anumolu, Peter Yim, Matthew L. Clarke, Hyeong G. Kang, Jeeseong C. Hwang
Rapid and quantitative techniques are essential to analyze soft multicomponent nanoparticle clusters. Here we combine electrospray differential mobility analysis (ES-DMA), which measures the size of the entire cluster, with transmission electron microscopy

Shell and ligand-dependent blinking of CdSe-based core/shell nanocrystals

July 7, 2010
Author(s)
Bonghwan Chon, Sung Jun Lim, Wonjung Kim, Hyeong G. Kang, Taiha Joo, Jeeseong C. Hwang, Seung Koo Shin
Blinking of zinc blende CdSe-based core/shell nanocrystals is studied as a function of shell materials and surface ligands. CdSe/ZnS, CdSe/ZnSe/ZnS and CdSe/CdS/ZnS core/shell nanocrystals are prepared by colloidal synthesis and six monolayers of larger

Monitoring Photothermally Excited Nanoparticles via Multimodal Microscopy

May 19, 2010
Author(s)
Matthew L. Clarke, Shin G. Chou, Jeeseong Hwang
Generation of heat using optically excited nanoparticles can be beneficial or detrimental depending on the application. Therefore, clinically applicable studies are being pursued in an effort to achieve safe practices of nanoparticle-induced hyperthermia