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Search Publications by: Michael Halter (Fed)

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Displaying 26 - 50 of 76

Large-scale Time-lapse Microscopy of Oct4 Expression in Human Embryonic Stem Cell Colonies

May 22, 2016
Author(s)
Kiran Bhadriraju, Michael Halter, Julien M. Amelot, Peter Bajcsy, Joe Chalfoun, Antoine Vandecreme, Barbara Mallon, Kye-yoon Park, John T. Elliott, Subhash Sista, Anne L. Plant
Identification of critical characteristics of therapeutically active cells, and quantification of those characteristics, are essential for assuring consistency and potency of cell therapy products. We have developed image analysis and visualization

Survey Statistics of Automated Segmentations Applied to Optical Imaging of Mammalian Cells

January 8, 2016
Author(s)
Peter Bajcsy, Antonio Cardone, Joe Chalfoun, Michael W. Halter, Derek Juba, Marcin Kociolek, Michael P. Majurski, Adele P. Peskin, Carl G. Simon Jr., Mylene H. Simon, Antoine Vandecreme, Anne L. Plant, Mary C. Brady
The goal of this survey paper is to overview cellular measurements using optical microscopy imaging followed by automated image segmentation. The cellular measurements of primary interest are taken from mammalian cells and their components. They are

High Resolution Surface Plasmon Resonance Imaging for Single Cells

November 30, 2014
Author(s)
Alexander Peterson, Michael Halter, Alessandro Tona, Anne Plant
Background Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However

Nonparametric Estimates of Drift and Diffusion Profiles via Fokker-Planck Algebra

October 13, 2014
Author(s)
Steven P. Lund, Michael W. Halter, Joseph B. Hubbard
Diffusion processes superimposed upon deterministic motion play a key role in understanding and controlling the transport of matter, energy, momentum, and even information in physics, chemistry, material science, biology, and communications technology

Automated Ranking of Stem Cell Colonies by Translating Biological Rules to Computational Models

September 20, 2014
Author(s)
Adele P. Peskin, Steven P. Lund, YaShian Li-Baboud, Michael W. Halter, Anne L. Plant, Peter Bajcsy
This paper addresses the problem of automating an image ranking process for stem cell colonies. We automate the manual process in a novel way: instead of fitting off-the-shelf image features and colony ranks to prediction models, we define a new feature

Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass

August 11, 2014
Author(s)
Michael W. Halter, Elianna Bier, Paul C. DeRose, Gregory A. Cooksey, Steven J. Choquette, Anne L. Plant, John T. Elliott
Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist for

Testing Equality of Cell Populations Based on Shape and Geodesic Distance

December 3, 2013
Author(s)
Robert C. Hagwood, Javier Bernal, Michael W. Halter, John T. Elliott
Image cytometry has emerged as a valuable in-vitro screening tool and advances in automated microscopy have made it possible to readily analyze large cellular populations of image data. A statistical procedure to study homogeneity of such data is addressed

Optical Coherence Tomography for Dimensional Metrology of Lab-on-a-chip devices

October 27, 2013
Author(s)
Darwin R. Reyes-Hernandez, Michael W. Halter, Jeeseong Hwang
Here we present a new metrology tool for the determination of the dimensions of channels from final product Lab-on-a-chip devices. Using Optical Coherence Tomography (OCT) areas of up to 12 mm x 12 mm can be imaged in less than 1 second, without the need

Measurement Uncertainty in Cell Image Segmentation Data Analysis

August 13, 2013
Author(s)
Jin Chu Wu, Michael W. Halter, Raghu N. Kacker, John T. Elliott, Anne L. Plant
Cell image segmentation is a part of quantitative studies regarding cell movement and cell behavior, and it plays a critical role in molecular biology and cellular biochemistry. Therefore, it is fundamentally important to evaluate the performance levels of

Boltzmann's H-Function and Diffusion Processes

May 15, 2013
Author(s)
Steven P. Lund, Joseph B. Hubbard, Michael W. Halter
There exists a generalization of Boltzmann's $H$-function that allows for non-uniformly populated stationary states which may exist far from thermodynamic equilibrium. Here we describe a method for obtaining a generalized or collective diffusion

Metrology for Lab-on-a-Chip Final-Product Devices

May 12, 2013
Author(s)
Darwin R. Reyes-Hernandez, Michael W. Halter, Jeeseong Hwang
New metrology tools to measure the critical parameters of internal structures in lab on a chip devices are greatly needed in order to develop standard tests for this technology. Here we present a method that combines a custom made optical coherence

Segmenting Time-lapse Phase Contrast Images of Adjacent NIH 3T3 Cells

January 15, 2013
Author(s)
Joe Chalfoun, Alden A. Dima, Marcin Kociolek, Michael W. Halter, Antonio Cardone, Adele P. Peskin, Peter Bajcsy, Mary C. Brady
We present a new method for segmenting phase contrast images of NIH 3T3 fibroblast cells that is accurate even when cells are in contact. The problem of segmentation, when cells are in contact, poses a challenge to the accurate automation of cell counting