Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Search Publications by: Michael J. Tarlov (Fed)

Displaying 51 - 75 of 122

Temperature Gradient Focusing of Matched and Partially Mismatched DNA/PNA Hybridizations

October 20, 2004

Author(s)

K M. Balss, David J. Ross, Michael J. Tarlov

The hybridization reactions of DNA with peptide nucleic acids (PNA) were monitored in real time with Temperature Gradient Focusing (TGF). Both ex situ and in situ reactions of a perfect complement and mismatched ssDNA targets with PNA were monitored by

DNA Displacement Assay Integrated into Microfluidic Channels

July 1, 2004

Author(s)

Rebecca A. Zangmeister, Michael J. Tarlov

This paper describes the development of a unique fluorescence based DNA diagnostic assay incorporated into a microfluidic channel that does not require labeling of the target sequence prior to analysis. The assay is based on the displacement of a short

Atmospheric Pressure Microplasmas for Modifying Sealed Microfluidic Devices

March 8, 2004

Author(s)

J K. Evju, P B. Howell, Laurie E. Locascio, Michael J. Tarlov, J J. Hickman

A new DC microdischarge technique for chemical modification of microchannel walls is reported. Our results show that strongly hydrophilic or hydrophobic properties are quickly imparted unto the walls of assembled polymer microchannels in atmospheric

Chiral Separations by Temperature Gradient Focusing

February 1, 2004

Author(s)

K M. Balss, Wyatt N. Vreeland, Karen W. Phinney, Michael J. Tarlov, David J. Ross

Temperature gradient focusing (TGF) involves application of a temperature gradient across a microchannel or capillary. With an appropriate buffer, the temperature gradient creates a gradient in both the electric field and the electrophoretic velocity

Quantitative Characterization of DNA Films by X-ray photoelectron spectroscopy

January 20, 2004

Author(s)

D Y. Petrovykh, H Y. Kimura-Suda, Michael J. Tarlov, L J. Whitman

Self-assembled films of thiolated poly(dT)25 single-stranded DNA (ssDNA) on gold areintroduced as a model system for quantitative characterization of DNA films by XPS. Weevaluate the applicability of a simple substrate-overlayer model for data analysis

Selective DNA Sensing Elements Integrated Into Microfluidic Channels

December 1, 2003

Author(s)

Rebecca A. Zangmeister, Michael J. Tarlov

This paper describes the development of a unique fluorescence based DNA diagnostic assay that does not require pre-labeling of the target oligomer prior to analysis. The assay uses polyacrylamide hydrogel plugs immobilized in microfluidic channels, and is

Thermolithographic Patterning of Sol-Gel Metal Oxides on Micro hot plate Sensing Arrays Using Organosilanes

September 1, 2003

Author(s)

N O. Savage, S V. Roberson, John G. Gillen, Michael J. Tarlov, Stephen Semancik

Sol-gel derived SnO2 and Fe2O3 were selectively deposited on elements of microhotplate (?HP) arrays. The silicon micromachined ?HP arrays contain heating elements (100 ?m x 100 ?m) that are electronically addressable and thermally isolated from each other

UV Graft Polymerization of Polyacrylamide Hydrogel Plugs in Microfluidic Channels

August 19, 2003

Author(s)

Rebecca A. Zangmeister, Michael J. Tarlov

It has been previously demonstrated that DNA, modified on the 5' end with an acrylamide functionality, can be incorporated into a polyacrylamide hydrogel matrix. These types of DNA containing gels have recently been spatially immobilized in plastic

Base-Dependent Competitive Adsorption of Single-Stranded DNA on Gold

July 30, 2003

Author(s)

H Y. Kimura-Suda, D Y. Petrovykh, Michael J. Tarlov, L J. Whitman

Aqueous solutions of single-stranded DNA probes (ssDNA) are widely used with gold surfaces in current biotechnology and emerging nanotechnology applications. Although recent studies have shown that different DNA bases interact differentlywith gold surfaces

Effect of Surface Wettability on Fast Transient Microboiling Behavior

July 22, 2003

Author(s)

O C. Thomas, Richard E. Cavicchi, Michael J. Tarlov

Microboiling events associated with the fast transient heating of a micrometer-scale metallic thin film heater immersed in water have been studied. The effect of surface properties on the microboiling transients was examined by modifying heater surfaces

Quantitative Analysis and Characterization of DNA Immobilized on Gold

April 30, 2003

Author(s)

D Y. Petrovykh, H Y. Kimura-Suda, L J. Whitman, Michael J. Tarlov

We describe the complementary use of x-ray photoelectron spectroscopy (XPS) and fourier transform infrared (FTIR) spectroscopy to quantitatively characterize the immobilization of thiolated poly(dT)25 single-stranded DNA (ssDNA) on gold. When electron

Structure of 1,4-Benzenedimethanethiol Self-Assembled Monolayers on Gold Grown by Solution and Vapor Techniques

April 29, 2003

Author(s)

D L. Pugmire, Michael J. Tarlov, Roger D. van Zee, Jawad Naciri

Characteristics of decanethiol and benzenedimethanethiol (BDMT) self-assembled monolayers (SAMs) grown using solution and vapor techniques were studied with X-ray photoelectron spectroscopy (XPS) and in-plane resistivity measurements. Self-assembled

DNA-Based Sensors

January 1, 2003

Author(s)

Michael J. Tarlov, A B. Steel

The development of DNA-based sensors for genetic assay and sequencing applications has exploded over the last decade. Nearly all of these devices rely on the hybridization of a complementary oligonucleotide with surface-confined, single-stranded DNA or RNA