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Search Publications by: John P. Marino (Fed)

Displaying 101 - 125 of 152

Examining Ty3 Polypurine Tract Structure and Function by Nucleoside Analog Interference

February 1, 2006

Author(s)

Chandravanu Dash, John Marino, Stuart F. Le Grice

Although retroviral and LTR-retrotransposon PPTs differ significantly in both their length and sequence, they are accurately removed from (+) RNA and (+) DNA by their cognate RTs, suggesting common structural features of these RNA/DNA hybrids contribute to

A Ribose Sugar Conformational Switch in the LTR-Retrotransposon Ty3 Polypurine Tract-Containing RNA/DNA Hybrid

November 30, 2005

Author(s)

Hye Y. Yi-Brunozzi, D M. Brabazon, Daniela Lener, Stuart F. LeGrice, John Marino

To probe structural features of a polypurine-tract (PPT) that mediate its specific recognition and processing, a model 20bp RNA/DNA duplex, which includes the full PPT sequence of the saccharomyces cerevisiae LTR-retrotransposon Ty3, has been investigated

Heterotrimeric G-protein alpha-Subunit Adopts a "Preactivated" Conformation When Associated with beta-Subunits

August 29, 2005

Author(s)

N G. Abdulaev, T Ngo, C Zhang, A Dinh, D M. Brabazon, K D. Ridge, John P. Marino

Bacterial Expression and One-Step purification of an isotope-labeled heterotremic G-protein a-subunit

May 1, 2005

Author(s)

John P. Marino, N G. Abdulaev, C Zhang, A Dinh, T Ngo, P N. Bryan, D M. Brabazon, K D. Ridge

Bacterial Expression and One-Step Purification of an Isotope-Labeled Heterotrimeric G-Protein alpha-Subunit

May 1, 2005

Author(s)

N G. Abdulaev, Xi-Cheng Zhang, Andy Dinh, T Ngo, Phillip Bryan, D M. Brabazon, John Marino, Kevin Ridge

Heterologous expression systems are often employed to generate sufficient quantities of isotope-labeled proteins for high-resolution NMR studies. Recently, the interaction between the prodomain region of subtilisin and an active, mutant form of the mature

Single Molecule Fluorescence Resonance Energy Transfer of a RNA Kissing Complex

January 27, 2005

Author(s)

Peter Yim, Xiaoyi Y. Zhang, E S. DeJong, J M. Carroll, John P. Marino, Lori S. Goldner

We used single molecular fluorescence resonance energy transfer (FRET) to understand the conformation of a RNA loop to loop complex, termed a kissing complex. The kissing complex formed by two Escherichia Coli (ColE1) plasmid-encoded transcripts, RNA I and

A Proton-Coupled Dynamic Switch in the HIV-1 Dimerization Initiation Site Kissing Complex

February 1, 2004

Author(s)

M Mihailescu, John Marino

In human immunodeficiency virus type 1 (HIV-1), the dimerization initiation site (DIS) is the sequence primarily responsible for initiating the non-covalent linkage of two homologous strands of genomic RNA during viral assembly. The DIS loop contains an

Homonuclear Hartmann-Hahn Transfer With Reduced Relaxation Losses by Use of the MOCCA-XY16 Multiple Pulse Sequence

January 1, 2004

Author(s)

J Furrer, F Kramer, John Marino, S J. Glaser, B. Luy

Proflavine Acts as a Rev Inhibitor by Targeting the High-Affinity Rev Binding Site of the Rev-Responsive Element of HIV-1

July 8, 2003

Author(s)

E S. DeJong, C Chang, M K. Gilson, John Marino

Rev is an essential regulatory HIV-1 protein that binds the Rev Responsive Element (RRE) within the env gene of the HIV-1 RNA genome, activating the switch between viral latency and active viral replication. Previously, we have shown that selective

Evidence for Structural Changes in Carboxyl-Terminal Peptides of Transducin Alpha-Subunit Upon Binding a Soluble Mimic of Light-Activated Rhodopsin

January 21, 2003

Author(s)

D M. Brabazon, N G. Abdulaev, John Marino, K D. Ridge

Although a high-resolution crystal structure for the ground state of rhodopsin is now available, portions of the cytoplasmic surface are not well resolved and the structural basis for the interaction of the cytoplasmic loops with the retinal G-protein

JE-TROSY: combined J- and TROSY-spectroscopy for the measurement of one-bond couplings in macromolecules

January 1, 2003

Author(s)

B. Luy, John Marino

Since the recent introduction of the use of residual dipolar couplings in high-resolution NMR studies of macromolecules, there has been a renewed interest in rapid, sensitive methods for the measurement of 15N- 1H} and 13C- 1H} one-bond couplings. For

Mechanism of Nucleocapsid Protein Catalyzed Structural Isomerization of the Dimerization Initiation Site of HIV-1

December 17, 2002

Author(s)

M J. Rist, John Marino

Dimerization of two homologous strands of genomic RNA is an essential feature of retroviral replication. In the human immunodeficiency virus type I (HIV-1), a conserved stem-loop sequence, the dimerization initiation site (DIS), has been identified as the

Observation of H-Bond Mediated 3h J H2H3 Coupling Constants Across Watson-Crick AU Base Pairs in RNA

October 1, 2002

Author(s)

B. Luy, U Richter, E S. DeJong, O W. Sorensen, John Marino

3hJ H2H3 trans-hydrogen bond scalar couplings have been observed for the first time in Watson-Crick AU base pairs in uniformly 15N-labeled RNA oligonucleotides using a new 2hJ NN-HNN-E.COSY experiment. The experiment, utilizes adenosine H2 (AH2) for