Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Search Publications by: John P. Marino (Fed)

Displaying 126 - 150 of 152

Observation of H-Bond Mediated 3h J H2H3 Coupling Constants Across Watson-Crick AU Base Pairs in RNA

October 1, 2002

Author(s)

B. Luy, U Richter, E S. DeJong, O W. Sorensen, John Marino

3hJ H2H3 trans-hydrogen bond scalar couplings have been observed for the first time in Watson-Crick AU base pairs in uniformly 15N-labeled RNA oligonucleotides using a new 2hJ NN-HNN-E.COSY experiment. The experiment, utilizes adenosine H2 (AH2) for

Fluorescent Nucleotide Base Analogs as Probes of Nucleic Acid Structure, Dynamics and Interactions

August 1, 2002

Author(s)

M J. Rist, John Marino

Highly fluorescent nucleotide base analogs provide sensitive probes for studying the structure, dynamics and interactions of nucleic acids. These analogs can be incorporated site-specifically into oligonucleotides through standard automated synthetic

RNA and RNA-Protein Complexes as Targets for Therapeutic Intervention

May 1, 2002

Author(s)

E S. DeJong, B. Luy, John Marino

Today, the majority of pharmaceuticals developed to treat cancers and viral/bacterial infections target cellular, bacterial or viral proteins known to be associated with a given pathology. Although proteins are the focus of most current drug discovery

1 H- 31 P CPMG-Correlated Experiments for the Assignment of Nucleic Acids

November 14, 2001

Author(s)

B. Luy, John Marino

Phosphorus (31P) nuclear magnetic resonance (NMR) spectroscopy can provide important information about the structure and dynamics of nucleic acids in solution. The application of 31P NMR, however, often requires the difficult task of resolving and

S 3 E-E.COSY Methods for the Measurement of 19 F Associated Scalar and Dipolar Coupling Constants

September 1, 2001

Author(s)

B. Luy, J J. Barchi, John Marino

A 1H-19F spin state selective excitation (S3E) pulse sequence element has been applied in combination with 1H homonuclear mixing to create E.COSY-type experiments designed to measure scalar JHF2' and JHH2' and residual dipolar DHF2' and DHH2' couplings in

Single Molecule Probes

August 1, 2001

Author(s)

Lori S. Goldner, K D. Weston, W F. Heinz, Jeeseong Hwang, E S. DeJong, John P. Marino

The technology to rapidly manipulate and screen individual molecules lies at the frontier of measurement science and impacts emerging bio- and nano-technologies. Fundamental biological and chemical processes can now be probed with unprecedented detail, one

Association of an RNA Kissing Complex Analyzed Using 2-Aminopurine Fluorescence

June 1, 2001

Author(s)

M J. Rist, John Marino

The fluorescent probe, 2-aminopurine-2'-O-methyl riboside (2-AP) has been selectively incorporated at adenosine positions in stem-loops (so called R1inv and R2inv), derived from the ColE1 plasmid encoded RNA I and RNA II transcripts, that interact to form

Measurement and Application of 1 H- 19 F Dipolar Couplings in the Structure Determination of 2'-Fluorolabeled RNA

May 1, 2001

Author(s)

B. Luy, John Marino

Residual dipolar couplings can provide powerful restraints for determination and refinement of the solution structure of macromolecules. The application of these couplings in nucleic acid structure elucidation can have an especially dramatic impact since

Direct Evidence for Watson-Crick Base Pairs in a Dynamic Region of RNA Structure

August 23, 2000

Author(s)

B. Luy, John Marino

Trans-H-bond 2hJ N1N3 scalar couplings were measured for the RNA hairpin, CopA29, which is derived from the R1 plasmid encoded RNA transcript, CopA, 21 using a dual-detected uracil H3/adenine-H2 (UH3/AH2) J nn HNN-COSY (see supporting Information for

Mechanism of Neomycin and Rev Peptide Binding to the Rev Responsive Element of HIV-1 as Determined by Fluorescence and NMR Spectroscopy

May 16, 2000

Author(s)

K Lacourciere, J T. Stivers, John Marino

Rev is an essential HIV-1 regulatory protein that binds the Rev Responsive Element (RRE) within the env gene of the HIV-1 RNA genome and is involved in transport of unspliced or partially spliced viral mRNA from the cell nucleus to the cytoplasm. Previous

J-Coupling Restraints in RNA Structure Determination

December 1, 1999

Author(s)

John P. Marino, H Schwalbe, C Griesinger

Methods for the Determination of Torsion Angle Restraints in Biomacromolecules

December 1, 1999

Author(s)

C Griesinger, M Hennig, John Marino, B Reif, C Richter, H Schwalbe

Determination of 3JC4',P Coupling Constants in Oligonucleotides by a Quantitative J Method

December 1, 1998

Author(s)

C Richter, B Reif, K H. W {omlat} rner, S Quant, John Marino, J Engels, C Griesinger, H Schwalbe